Quantitative rt pcr protocol sybr green i 5 3 running 3 agarose gel after qrt pcr 4 analyze qrt pcr data. In a one step rt pcr protocol multiscribe reverse transcriptase and rnase inhibitor are added to the sybr green pcr master mix. Powerup sybr green master mix provides robust results from 100 ng to 0 1 pg cdna per reaction.
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